聯絡電話
陳定濰
助理教授
高通量系統基因體學
肝癌與分子醫學
肝腎代謝疾病的動物模式
- 電子郵件信箱
dennis8857@gmail.com
研究簡介
惡性肝腫瘤，尤其是肝細胞癌 (hepatocellular carcinoma)，是全球疾病死亡的主要因素之一。 腫瘤細胞與其周圍細胞之間的相互作用和溝通形成腫瘤微環境 (Tumor microenvironments)，對於造成局部侵襲和遠處轉移至關重要。 在腫瘤發展過程中，免疫抑制因子從癌細胞中釋放，伴隨著肝腫瘤的強烈免疫致發生特徵，是逃避腫瘤免疫監視的關鍵，並限制了免疫療法的治療效益。具體而言，目前已知自噬作用 (Autophagy) 在腫瘤微環境中扮演主要角色，其在免疫抑制和促進腫瘤惡性化的機制上常有相互矛盾的結果。肝細胞癌進展和轉移與自噬作用的關係，仍是日益受到關注的研究領域。
我們近期的臨床和實驗證據顯示，肝癌細胞的自噬抑制可能有助於加速腫瘤的生長和轉移的進行，其微環境的探討對於進一步的預防和治療非常重要。因此，我們從基礎理論上將脈管系統，炎症控制，凝血和免疫力的角色，作為改善肝臟微環境以預防及治療肝癌的研究目標，並著重於與自噬作用相關的分子事件（靶標與調節因子）。在過去兩年中，我們特別研究了高表達凝血因子 Factor VII 的肝腫瘤組織中，微小核醣核酸 miR-135a 促進肝細胞癌進展的分子機制。
我們也進一步分析 Factor VII 促進復發和轉移的機制，其可能參與一系列促發生長、侵襲和調節上皮−間質轉化可塑性 (Epithelial-to-Mesenchymal Transition, EMT Plasticity) 功能的訊號傳遞路徑。
凝血因子 Factor VII 訊號傳遞引發的自噬抑制導因於 miR-135a 的過度表達，與肝腫瘤惡性化高度相關。
研究證實肝腫瘤中 Factor VII 和 miR-135a 的表達存在顯著的正相關，且 miR-135a 和自噬蛋白 ATG14 表達量有顯著負相關。與鄰近正常肝組織區域相比，腫瘤具有高表達 miR-135a 的肝癌患者，其無疾病存活率 (disease-free survival) 遠低於腫瘤中低 miR-135a 表達的患者。
肝癌細胞實驗進一步證實，自噬蛋白ATG14是miR-135a的直接抑制標的，並進一步促進肝癌細胞的生長與侵襲能力。
凝血因子Factor VII引發一系列miRNA和mRNA基因的差異表達，在肝癌的腫瘤組織中主導調節癌細胞上皮−間質轉化的功能。
我們進一步發現肝腫組織中，80%與Factor VII表達量相關的miRNAs與上皮−間質轉化的功能有關，其中的77%(75/93, 包括miR-135a)與Factor VII表達量顯著正相關。這些可能參與上皮−間質轉化過程的基因，與細胞粘附(cell adhesion)、細胞凋亡(apoptosis)和免疫反應(immune responses)的負向調節有關。
學術著作
Recent publications (Pubmed Search)
1: Yeh YT, Chen KD, Huang CH, Tsai JR, Kuo HC. Eggerthella lenta down
regulated flavone and flavonol biosynthesis promoted Kawasaki disease.
Virulence. 2025 Dec;16(1):2512401. doi: 10.1080/21505594.2025.2512401. Epub 2025
May 31. PMID: 40448518; PMCID: PMC12128670.
2: Guo MM, Chen KD, Kuo HC. Semaphorin 7a Regulates the Expression of IL-4 and
IL-33 in a Cell Model of Atopic Dermatitis and Is Associated With Disease
Severity. Exp Dermatol. 2025 Mar;34(3):e70087. doi: 10.1111/exd.70087. PMID:
40129061.
3: Chen CC, Hsu LW, Chen KD, Chiu KW, Kung CP, Li SR, Goto S, Chen CL, Huang KT.
Extracellular calreticulin regulates fibrogenic and immunogenic properties of
hepatic stellate cells. Int Immunopharmacol. 2025 Feb 20;148:114129. doi:
10.1016/j.intimp.2025.114129. Epub 2025 Jan 24. PMID: 39862632.
4: Shih WL, Yeh TM, Chen KD, Leu S, Liu SF, Huang YH, Kuo HC. Hydrogen Gas
Inhalation Treatment for Coronary Artery Lesions in a Kawasaki Disease Mouse
Model. Life (Basel). 2024 Jun 24;14(7):796. doi: 10.3390/life14070796. PMID:
39063551; PMCID: PMC11277616.
5: Chen CC, Hsu LW, Chen KD, Chiu KW, Kung CP, Li SR, Chen CL, Huang KT.
Calreticulin regulates hepatic stellate cell activation through modulating TGF-
beta-induced Smad signaling. Cell Calcium. 2024 Jul;121:102895. doi:
10.1016/j.ceca.2024.102895. Epub 2024 Apr 30. PMID: 38703416.
6: Shih WL, Yeh TM, Chen KD, Leu S, Kuo HC. Positive Echocardiographic
Association between Carotid Artery and Coronary Artery Diameter and Z-Score in a
Mouse Model of Kawasaki Disease. Diagnostics (Basel). 2024 Jan 8;14(2):145. doi:
10.3390/diagnostics14020145. PMID: 38248023; PMCID: PMC10814724.
7: Kuo HC, Chen KD, Li PC. Molecular Hydrogen: Emerging Treatment for Stroke
Management. Chem Res Toxicol. 2023 Dec 18;36(12):1864-1871. doi:
10.1021/acs.chemrestox.3c00259. Epub 2023 Nov 21. PMID: 37988743.
8: Chang HC, Liu SF, Kuo HC, Chen KD, Liu JF, Tseng CW, Weng CM, Chou TC.
Smoking cessation and influenza vaccination can reduce the healthcare burden of
COPD. Tob Induc Dis. 2023 Aug 26;21:108. doi: 10.18332/tid/167962. PMID:
37637228; PMCID: PMC10458000.
9: Chen KD, Huang YH, Wu WS, Chang LS, Chu CL, Kuo HC. Comparable bidirectional
neutrophil immune dysregulation between Kawasaki disease and severe COVID-19.
Front Immunol. 2022 Sep 8;13:995886. doi: 10.3389/fimmu.2022.995886. PMID:
36159873; PMCID: PMC9499176.
10: Chang LS, Chen KD, Huang YH, Kuo HC. Expression of Eosinophilic Subtype
Markers in Patients with Kawasaki Disease. Int J Mol Sci. 2022 Sep
3;23(17):10093. doi: 10.3390/ijms231710093. PMID: 36077487; PMCID: PMC9456171.
11: Lin SH, Wu KT, Wang CC, Huang KT, Chen KD, Hsu LW, Eng HL, Chiu KW. Liver
Graft MicroRNAs Expression in Different Etiology of Acute Jaundice after Living
Donor Liver Transplantation. Biology (Basel). 2022 Aug 17;11(8):1228. doi:
10.3390/biology11081228. PMID: 36009855; PMCID: PMC9404977.
12: Huang DS, Liu TT, Lu WT, Wang CC, Lin CC, Yong CC, Chen KD, Liu YW, Kuo YH,
Yen YH, Hu TH, Tsai MC. Comparison of portal and capsular microscopic vascular
invasion in the outcomes of early HCC after curative resection. Am J Cancer Res.
2022 Jun 15;12(6):2659-2672. PMID: 35812043; PMCID: PMC9251683.
13: Yang YL, Kuo HC, Chen KD, Chu CH, Kuo KC, Guo MM, Chang LS, Huang YH.
Combination of Hemoglobin-for-Age Z-Score and Plasma Hepcidin Identified as a
Novel Predictor for Kawasaki Disease. Children (Basel). 2022 Jun 18;9(6):913.
doi: 10.3390/children9060913. PMID: 35740850; PMCID: PMC9222120.
14: Lin SH, Wang CC, Huang KT, Chen KD, Hsu LW, Eng HL, Chiu KW. Liver Graft
Pathology and Low Serum 25-Hydroxyvitamin D after Living Donor Liver
Transplantation. Metabolites. 2022 Apr 25;12(5):388. doi:
10.3390/metabo12050388. PMID: 35629892; PMCID: PMC9147938.
15: Wu WS, Yang TH, Chen KD, Lin PH, Chen GR, Kuo HC. KDmarkers: A biomarker
database for investigating epigenetic methylation and gene expression levels in
Kawasaki disease. Comput Struct Biotechnol J. 2022 Mar 10;20:1295-1305. doi:
10.1016/j.csbj.2022.02.032. PMID: 35356542; PMCID: PMC8931344.
16: Huang YH, Chen KD, Kuo KC, Guo MM, Chang LS, Yang YL, Kuo HC. Human
Transcriptome Array Analysis Identifies CDR2 as a Novel Suppressed Gene for
Kawasaki Disease. Diagnostics (Basel). 2022 Jan 19;12(2):240. doi:
10.3390/diagnostics12020240. PMID: 35204331; PMCID: PMC8871175.
17: Guo MM, Huang YH, Wang FS, Chang LS, Chen KD, Kuo HC. CD36 is Associated
With the Development of Coronary Artery Lesions in Patients With Kawasaki
Disease. Front Immunol. 2022 Jan 27;13:790095. doi: 10.3389/fimmu.2022.790095.
PMID: 35154107; PMCID: PMC8828496.
18: Chen CC, Hsu LW, Chen KD, Chiu KW, Chen CL, Huang KT. Emerging Roles of
Calcium Signaling in the Development of Non-Alcoholic Fatty Liver Disease. Int J
Mol Sci. 2021 Dec 27;23(1):256. doi: 10.3390/ijms23010256. PMID: 35008682;
PMCID: PMC8745268.
19: Lin SH, Wu KT, Wang CC, Huang KT, Chen KD, Lin CC, Hsu LW, Chiu KW. HCV RNA
in serum and liver samples of patients undergoing living donor liver
transplantation. J Int Med Res. 2021 Aug;49(8):3000605211034945. doi:
10.1177/03000605211034945. PMID: 34344219; PMCID: PMC8358508.
20: Chen KD, Huang YH, Guo MM, Chang LS, Chu CH, Bu LF, Chu CL, Lee CH, Liu SF,
Kuo HC. DNA Methylation Array Identifies Golli-MBP as a Biomarker for Disease
Severity in Childhood Atopic Dermatitis. J Invest Dermatol. 2022
Jan;142(1):104-113. doi: 10.1016/j.jid.2021.06.025. Epub 2021 Jul 19. PMID:
34293355.
21: Chang LS, Chen YJ, Huang PY, Chen KD, Lo MH, Huang YH, Guo MM, Kuo HC.
Significantly Lower Immunoglobulin M Levels 6 Months After Disease Onset in
Patients With Kawasaki Disease With Coronary Artery Lesions. J Am Heart Assoc.
2021 Jun 15;10(12):e020505. doi: 10.1161/JAHA.120.020505. Epub 2021 Jun 5. PMID:
34096327; PMCID: PMC8477878.
22: Chang LS, Yu HR, Chu CL, Chen KD, Huang YH, Guo MM, Weng KP, Kuo HC. Long-
Term Hypermethylation of FcγR2B in Leukocytes of Patients with Kawasaki
Disease. J Clin Med. 2021 May 27;10(11):2347. doi: 10.3390/jcm10112347. PMID:
34071896; PMCID: PMC8199050.
23: Chen KD, Lin WC, Kuo HC. Chemical and Biochemical Aspects of Molecular
Hydrogen in Treating Kawasaki Disease and COVID-19. Chem Res Toxicol. 2021 Apr
19;34(4):952-958. doi: 10.1021/acs.chemrestox.0c00456. Epub 2021 Mar 15. PMID:
33719401.
24: Cho WR, Wang CC, Tsai MY, Chou CK, Liu YW, Wu YJ, Lin MT, Chen KD, Chuang
CH, Huang PY, Hu TH, Tsai MC. Impact of metformin use on the recurrence of
hepatocellular carcinoma after initial liver resection in diabetic patients.
PLoS One. 2021 Mar 4;16(3):e0247231. doi: 10.1371/journal.pone.0247231. PMID:
33661912; PMCID: PMC7932176.
25: Chen KD, Hsu CN, Wu YJ, Chu CH, Huang KT, Tsai MC, Chiu KW, Cheng BC, Chiu
CH, Chen CL, Lin CC. The Perioperatively Altered Neutrophil-to-Lymphocyte Ratio
Associates with Impaired DNA Damage Response in Liver Transplantation Recipients
with Hepatocellular Carcinoma. Diagnostics (Basel). 2021 Jan 30;11(2):209. doi:
10.3390/diagnostics11020209. PMID: 33573309; PMCID: PMC7912615.
26: Yang SY, Wang CC, Chen KD, Liu YW, Lin CC, Chuang CH, Tsai YC, Yao CC, Yen
YH, Hsiao CC, Hu TH, Tsai MC. Statin use is associated with a lower risk of
recurrence after curative resection in BCLC stage 0-A hepatocellular carcinoma.
BMC Cancer. 2021 Jan 15;21(1):70. doi: 10.1186/s12885-021-07796-7. PMID:
33446127; PMCID: PMC7808883.
27: Hsu LW, Huang KT, Nakano T, Chiu KW, Chen KD, Goto S, Chen CL. MicroRNA-301a
inhibition enhances the immunomodulatory functions of adipose-derived
mesenchymal stem cells by induction of macrophage M2 polarization. Int J
Immunopathol Pharmacol. 2020 Jan-Dec;34:2058738420966092. doi:
10.1177/2058738420966092. PMID: 33121303; PMCID: PMC7607751.
28: Cho WR, Hung CH, Chen CH, Lin CC, Wang CC, Liu YW, Wu YJ, Yong CC, Chen KD,
Tsai YC, Hu TH, Tsai MC. Ability of the post-operative ALBI grade to predict the
outcomes of hepatocellular carcinoma after curative surgery. Sci Rep. 2020 Apr
29;10(1):7290. doi: 10.1038/s41598-020-64354-0. PMID: 32350365; PMCID:
PMC7190718.
29: Tsai MC, Liu YY, Lin CC, Wang CC, Wu YJ, Yong CC, Chen KD, Chuah SK, Yao CC,
Huang PY, Chen CH, Hu TH, Chen CL. Gut Microbiota Dysbiosis in Patients with
Biopsy-Proven Nonalcoholic Fatty Liver Disease: A Cross-Sectional Study in
Taiwan. Nutrients. 2020 Mar 19;12(3):820. doi: 10.3390/nu12030820. PMID:
32204538; PMCID: PMC7146257.
30: Huang KT, Chen KD, Hsu LW, Kung CP, Li SR, Chen CC, Chiu KW, Goto S, Chen
CL. Decreased PEDF Promotes Hepatic Fatty Acid Uptake and Lipid Droplet
Formation in the Pathogenesis of NAFLD. Nutrients. 2020 Jan 20;12(1):270. doi:
10.3390/nu12010270. PMID: 31968655; PMCID: PMC7019565.
31: Huang PY, Wang CC, Lin CC, Lu SN, Wang JH, Hung CH, Kee KM, Chen CH, Chen
KD, Hu TH, Tsai MC. Predictive Effects of Inflammatory Scores in Patients with
BCLC 0-A Hepatocellular Carcinoma after Hepatectomy. J Clin Med. 2019 Oct
14;8(10):1676. doi: 10.3390/jcm8101676. PMID: 31614976; PMCID: PMC6832545.
32: Huang YH, Chen KD, Lo MH, Cai XY, Chang LS, Kuo YH, Huang WD, Kuo HC.
Decreased DNA methyltransferases expression is associated with coronary artery
lesion formation in Kawasaki disease. Int J Med Sci. 2019 Apr 25;16(4):576-582.
doi: 10.7150/ijms.32773. PMID: 31171909; PMCID: PMC6535659.
33: Huang YH, Chen KD, Lo MH, Cai XY, Kuo HC. Decreased Steroid Hormone Receptor
NR4A2 Expression in Kawasaki Disease Before IVIG Treatment. Front
Pediatr. 2019 Feb 4;7:7. doi: 10.3389/fped.2019.00007. PMID: 30778379; PMCID:
PMC6369254.
34: Huang KT, Hsu LW, Chen KD, Kung CP, Goto S, Chen CL. Decreased PEDF
Expression Promotes Adipogenic Differentiation through the Up-Regulation of
CD36. Int J Mol Sci. 2018 Dec 11;19(12):3992. doi: 10.3390/ijms19123992. PMID:
30544997; PMCID: PMC6321369.
35: Chen KD, Lin CC, Tsai MC, Huang KT, Chiu KW. Tumor microenvironment mediated
by suppression of autophagic flux drives liver malignancy. Biomed J. 2018
Jun;41(3):163-168. doi: 10.1016/j.bj.2018.03.002. Epub 2018 Jun 29. PMID:
30080656; PMCID: PMC6138774.
36: Chen KD, Huang YH, Ming-Huey Guo M, Lin TY, Weng WT, Yang HJ, Yang KD, Kuo
HC. The human blood DNA methylome identifies crucial role of β-catenin in the
pathogenesis of Kawasaki disease. Oncotarget. 2018 Jun 19;9(47):28337-28350.
doi: 10.18632/oncotarget.25305. PMID: 29983864; PMCID: PMC6033340.
37: Huang KT, Lin CC, Tsai MC, Chen KD, Chiu KW. Pigment epithelium-derived
factor in lipid metabolic disorders. Biomed J. 2018 Apr;41(2):102-108. doi:
10.1016/j.bj.2018.02.004. PMID: 29866598; PMCID: PMC6138776.
38: Chiu KW, Nakano T, Hu TH, Chen KD, Hsu LW, Eng HL, Cheng YF, Goto S, Chen
CL. Association between Subclinical Low Serum 25(OH)D in Donors and Fatty Liver
Disease in Recipients after Living Donor Liver Transplantation. Biomed Res Int.
2018 Mar 19;2018:4508085. doi: 10.1155/2018/4508085. PMID: 29750155; PMCID:
PMC5884236.
39: Chiu KW, Nakano T, Chen KD, Hu TH, Lin CC, Hsu LW, Chen CL, Goto S.
Identification of IL-28B Genotype Modification in Hepatocytes after Living Donor
Liver Transplantation by Laser Capture Microdissection and Pyrosequencing
Analysis. Biomed Res Int. 2018 Mar 4;2018:1826140. doi: 10.1155/2018/1826140.
PMID: 29686997; PMCID: PMC5857340.
40: Huang KT, Kuo IY, Tsai MC, Wu CH, Hsu LW, Chen LY, Kung CP, Cheng YF, Goto
S, Chou YW, Chen CL, Lin CC, Chen KD. Factor VII-Induced MicroRNA-135a Inhibits
Autophagy and Is Associated with Poor Prognosis in Hepatocellular Carcinoma. Mol
Ther Nucleic Acids. 2017 Dec 15;9:274-283. doi: 10.1016/j.omtn.2017.10.002. Epub
2017 Oct 7. PMID: 29246306; PMCID: PMC5675721.
41: Chen YC, Lin AS, Hung YC, Chen KD, Wu CY, Lie CH, Hsiao CC, Chen CJ, Liu SF,
Fang WF, Chang JC, Wang TY, Wang YH, Chung YH, Chao TY, Leung SY, Su MC, Lin MC.
Whole genome gene expression changes and hematological effects of rikkunshito in
patients with advanced non-small cell lung cancer receiving first line
chemotherapy. Exp Ther Med. 2017 Sep;14(3):2040-2052. doi:
10.3892/etm.2017.4773. Epub 2017 Jul 11. PMID: 28962123; PMCID: PMC5609175.
42: Chen YC, Chen KD, Su MC, Chin CH, Chen CJ, Liou CW, Chen TW, Chang YC, Huang
KT, Wang CC, Wang TY, Chang JC, Lin YY, Zheng YX, Lin MC, Hsiao CC. Genome-wide
gene expression array identifies novel genes related to disease severity and
excessive daytime sleepiness in patients with obstructive sleep apnea. PLoS One.
2017 May 17;12(5):e0176575. doi: 10.1371/journal.pone.0176575. PMID: 28520763;
PMCID: PMC5435176.
43: Chen YC, Lin MC, Hsiao CC, Zheng YX, Chen KD, Sung MT, Chen CJ, Wang TY, Lin
YY, Chang HC, Chen YM, Chang JC. Increased S100A15 expression and decreased DNA
methylation of its gene promoter are involved in high metastasis potential and
poor outcome of lung adenocarcinoma. Oncotarget. 2017 Jul 11;8(28):45710-45724.
doi: 10.18632/oncotarget.17391. PMID: 28498804; PMCID: PMC5542220.
44: Chen CC, Hsu LW, Nakano T, Huang KT, Chen KD, Lai CY, Goto S, Chen CL. DHL-
HisZn, a novel antioxidant, enhances adipogenic differentiation and
antioxidative response in adipose-derived stem cells. Biomed Pharmacother. 2016
Dec;84:1601-1609. doi: 10.1016/j.biopha.2016.10.066. Epub 2016 Nov 5. PMID:
27825800.
45: Chiu KW, Nakano T, Chen KD, Lin CC, Hu TH, Goto S, Chen CL. Association of
IL28B SNPs rs12979860 and rs8099917 on Hepatitis C Virus-RNA Status in
Donors/Recipients of Living Donor Liver Transplantation. PLoS One. 2016 Jun
8;11(6):e0156846. doi: 10.1371/journal.pone.0156846. PMID: 27275739; PMCID:
PMC4898820.
46: Chen YC, Chen TW, Su MC, Chen CJ, Chen KD, Liou CW, Tang P, Wang TY, Chang
JC, Wang CC, Lin HC, Chin CH, Huang KT, Lin MC, Hsiao CC. Whole Genome DNA
Methylation Analysis of Obstructive Sleep Apnea: IL1R2, NPR2, AR, SP140
Methylation and Clinical Phenotype. Sleep. 2016 Apr 1;39(4):743-55. doi:
10.5665/sleep.5620. PMID: 26888452; PMCID: PMC4791608.
47: Chen KD, Huang KT, Lin CC, Weng WT, Hsu LW, Goto S, Nakano T, Lai CY, Kung
CP, Chiu KW, Wang CC, Cheng YF, Ma YY, Chen CL. MicroRNA-27b Enhances the
Hepatic Regenerative Properties of Adipose-Derived Mesenchymal Stem Cells. Mol
Ther Nucleic Acids. 2016 Feb 2;5(2):e285. doi: 10.1038/mtna.2015.55. PMID:
26836372; PMCID: PMC4884788.
48: Chiu KW, Nakano T, Chen KD, Hsu LW, Lai CY, Huang CY, Cheng YF, Goto S, Chen
CL. Repeated-measures implication of hepatocellular carcinoma biomarkers in
living donor liver transplantation. PLoS One. 2015 May 15;10(5):e0124943. doi:
10.1371/journal.pone.0124943. PMID: 25978323; PMCID: PMC4433208.
49: Chiu KW, Nakano T, Chen KD, Hsu LW, Lai CY, Huang CY, Cheng YF, Goto S, Chen
CL. Cytochrome P450 in living donor liver transplantation. J Biomed Sci. 2015
May 15;22(1):32. doi: 10.1186/s12929-015-0140-4. PMID: 25975271; PMCID:
PMC4432787.
50: Hsu LW, Nakano T, Huang KT, Chen CC, Chen KD, Lai CY, Yang SM, Lin CC, Wang
CC, Cheng YF, Chiu KW, Kuo YR, Goto S, Chen CL. Prolonged survival by combined
treatment with granulocyte colony-stimulating factor and dipeptidyl peptidase IV
inhibitor in a rat small-for-size liver transplantation model. Hepatol Res. 2015
Jul;45(7):804-13. doi: 10.1111/hepr.12413. Epub 2014 Oct 23. PMID: 25179290.
51: Nakano T, Goto S, Lai CY, Hsu LW, Tseng HP, Chen KD, Chiu KW, Wang CC, Cheng
YF, Chen CL. Induction of antinuclear antibodies by de novo autoimmune hepatitis
regulates alloimmune responses in rat liver transplantation. Clin Dev Immunol.
2013;2013:413928. doi: 10.1155/2013/413928. Epub 2013 Dec 23. PMID: 24454474;
PMCID: PMC3886613.
52: Chiu KW, Nakano T, Chen KD, Hsu LW, Lai CY, Chiu HC, Huang CY, Cheng YF,
Goto S, Chen CL. Homogeneous phenomenon of the graft when using different
genotype characteristic of recipients/donors in living donor liver
transplantation. World J Hepatol. 2013 Nov 27;5(11):642-8. doi:
10.4254/wjh.v5.i11.642. PMID: 24303093; PMCID: PMC3847948.
53: Loke SS, Yang KD, Chen KD, Chen JF. Erosive esophagitis associated with
metabolic syndrome, impaired liver function, and dyslipidemia. World J
Gastroenterol. 2013 Sep 21;19(35):5883-8. doi: 10.3748/wjg.v19.i35.5883. PMID:
24124334; PMCID: PMC3793143.
54: Chiu KW, Nakano T, Chen KD, Lai CY, Hsu LW, Chiu HC, Huang CY, Cheng YF,
Goto S, Chen CL. Pyrosequencing to identify homogeneous phenomenon when using
recipients/donors with different CYP3A5*3 genotypes in living donor liver
transplantation. PLoS One. 2013 Aug 8;8(8):e71314. doi:
10.1371/journal.pone.0071314. PMID: 23951129; PMCID: PMC3738598.
55: Chiu KW, Hu TH, Nakano T, Chen KD, Lai CY, Hsu LW, Tseng HP, Chiu HC, Cheng
YF, Goto S, Chen CL. Biological interactions of CYP2C19 genotypes with
CYP3A4*18, CYP3A5*3, and MDR1-3435 in living donor liver transplantation
recipients. Transplant Res. 2013 Apr 23;2(1):6. doi: 10.1186/2047-1440-2-6.
PMID: 23617933; PMCID: PMC3643843.
56: Chen KD, Goto S, Hsu LW, Lin TY, Nakano T, Lai CY, Chang YC, Weng WT, Kuo
YR, Wang CC, Cheng YF, Ma YY, Lin CC, Chen CL. Identification of miR-27b as a
novel signature from the mRNA profiles of adipose-derived mesenchymal stem cells
involved in the tolerogenic response. PLoS One. 2013 Apr 16;8(4):e60492. doi:
10.1371/journal.pone.0060492. PMID: 23613728; PMCID: PMC3628792.
57: Chen YC, Hsiao CC, Chen KD, Hung YC, Wu CY, Lie CH, Liu SF, Sung MT, Chen
CJ, Wang TY, Chang JC, Tang P, Fang WF, Wang YH, Chung YH, Chao TY, Leung SY, Su
MC, Wang CC, Lin MC. Peripheral immune cell gene expression changes in advanced
non-small cell lung cancer patients treated with first line combination
chemotherapy. PLoS One. 2013;8(2):e57053. doi: 10.1371/journal.pone.0057053.
Epub 2013 Feb 25. PMID: 23451142; PMCID: PMC3581559.
58: Nakano T, Lai CY, Goto S, Hsu LW, Kawamoto S, Ono K, Chen KD, Lin CC, Chiu
KW, Wang CC, Cheng YF, Chen CL. Immunological and regenerative aspects of
hepatic mast cells in liver allograft rejection and tolerance. PLoS One.
2012;7(5):e37202. doi: 10.1371/journal.pone.0037202. Epub 2012 May 15. PMID:
22615941; PMCID: PMC3352886.
59: Hsu LW, Goto S, Nakano T, Chen KD, Wang CC, Lai CY, Hou CH, Chang YC, Cheng
YF, Chiu KW, Chen CC, Chen SH, Chen CL. The effect of exogenous histone H1 on
rat adipose-derived stem cell proliferation, migration, and osteogenic
differentiation in vitro. J Cell Physiol. 2012 Oct;227(10):3417-25. doi:
10.1002/jcp.24042. PMID: 22223405.
60: Ma YY, Lin H, Moh JS, Chen KD, Wang IW, Ou YC, You YS, Lung CC. Low-dose
LBH589 increases the sensitivity of cisplatin to cisplatin-resistant ovarian
cancer cells. Taiwan J Obstet Gynecol. 2011 Jun;50(2):165-71. doi:
10.1016/j.tjog.2011.01.022. PMID: 21791302.
61: Chen KD, Chang PT, Ping YH, Lee HC, Yeh CW, Wang PN. Gene expression
profiling of peripheral blood leukocytes identifies and validates ABCB1 as a
novel biomarker for Alzheimer’s disease. Neurobiol Dis. 2011 Sep;43(3):698-705.
doi: 10.1016/j.nbd.2011.05.023. Epub 2011 Jun 6. PMID: 21669286.
62: Chen KD, Hsu LW, Goto S, Yeh CW, Nakano T, Lai CY, Chang YC, Hou CH, Wang
CC, Cheng YF, Chiu KW, Lin CC, Chen CL. Adaptor protein Shc acts as an immune-
regulator for the LPS-stimulated maturation of bone marrow-derived dendritic
cells. BMC Immunol. 2011 May 25;12:32. doi: 10.1186/1471-2172-12-32. PMID:
21612602; PMCID: PMC3123310.
63: Nakano T, Cheng YF, Lai CY, Hsu LW, Chang YC, Deng JY, Huang YZ, Honda H,
Chen KD, Wang CC, Chiu KW, Jawan B, Eng HL, Goto S, Chen CL. Impact of
artificial sunlight therapy on the progress of non-alcoholic fatty liver disease
in rats. J Hepatol. 2011 Aug;55(2):415-25. doi: 10.1016/j.jhep.2010.11.028. Epub
2010 Dec 22. PMID: 21184788.
64: Tsai MS, Hwang SM, Chen KD, Lee YS, Hsu LW, Chang YJ, Wang CN, Peng HH,
Chang YL, Chao AS, Chang SD, Lee KD, Wang TH, Wang HS, Soong YK. Functional
network analysis of the transcriptomes of mesenchymal stem cells derived from
amniotic fluid, amniotic membrane, cord blood, and bone marrow. Stem Cells. 2007
Oct;25(10):2511-23. doi: 10.1634/stemcells.2007-0023. Epub 2007 Jun 7. PMID:
17556597.
65: Wang Y, Chang J, Chen KD, Li S, Li JY, Wu C, Chien S. Selective adapter
recruitment and differential signaling networks by VEGF vs. shear stress. Proc
Natl Acad Sci U S A. 2007 May 22;104(21):8875-9. doi: 10.1073/pnas.0703088104.
Epub 2007 May 11. PMID: 17496149; PMCID: PMC1885595.
66: Li S, Lao J, Chen BP, Li YS, Zhao Y, Chu J, Chen KD, Tsou TC, Peck K, Chien
S. Genomic analysis of smooth muscle cells in 3-dimensional collagen matrix.
FASEB J. 2003 Jan;17(1):97-9. doi: 10.1096/fj.02-0256fje. Epub 2002 Nov 15.
Erratum in: FASEB J. 2003 Jul;17(10):1376. PMID: 12475912.
67: Labrador V, Chen KD, Li YS, Muller S, Stoltz JF, Chien S. Interactions of
mechanotransduction pathways. Biorheology. 2003;40(1-3):47-52. PMID: 12454386.
68: Chang WM, Chen KD, Chen LY, Lai MT, Lai YK. Mitochondrial calcium-mediated
reactive oxygen species are essential for the rapid induction of the grp78 gene
in 9L rat brain tumour cells. Cell Signal. 2003 Jan;15(1):57-64. doi:
10.1016/s0898-6568(02)00055-4. PMID: 12401520.
69: Wang Y, Miao H, Li S, Chen KD, Li YS, Yuan S, Shyy JY, Chien S. Interplay
between integrins and FLK-1 in shear stress-induced signaling. Am J Physiol Cell
Physiol. 2002 Nov;283(5):C1540-7. doi: 10.1152/ajpcell.00222.2002. PMID:
12372815.
研究團隊
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卜莉鳳研究助理
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凃怡婷研究助理
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學術履歷
EDUCATION
Doctor of Philosophy, Life Sciences 1991-1997
Institute of Life Science
National Tsing Hua University, Hsinchu, Taiwan
Bachelor of Science, Microbiology 1987-1991
Department of Microbiology
Soochow University, Taipei, Taiwan
RESEARCH EXPERIENCE
Assistant Research Fellow 2009-present
Institute for Translational Research in Biomedicine
Kaohsiung Chang Gung Memorial Hospital, Kaohsiung, Taiwan
Assistant Professor 2005-2007
Department of Life Science
Chang Gung University, Tao-Yuan, Taiwan
Associate Director 2004-2005
Bioinformatics and Information Technology
Vita Genomics, Inc., Taipei, Taiwan
Research Fellow 2002-2005
Bioinformatics and Information Technology
Vita Genomics, Inc., Taipei, Taiwan
Adjunct Assistant Professor 2002-2005
Department of Microbiology
Soochow University, Taipei, Taiwan
Postdoctoral Fellow 2000-2001
Bioengineering Department
University of California, San Diego, San Diego, USA