其他研究資源

We offer a variety of preclinical imaging facilities, including 9.4T animal MRI, bioluminescence and fluorescence imaging system (IVIS), and animal ultrasound imaging system.

• Diffusion MRI (including diffusion tensor imaging and diffusion weighted imaging)
• Functional MRI and BOLD MR imaging
• Chemical exchange saturation transfer (CEST) MRI
• MR spectrum for metabolites (including  1 H and X nuclear)
• Wideband magnetic resonance imaging (WB-MRI) for high spatio-temporal MR imaging
• Quantitative susceptibility mapping (QSM) algorithm for quantifying magnetic properties and
  overcome non-locality of the magnetic field distribution from phase data
• In vivo optical imaging, including bioluminescence and fluorescence imaging
• 2D or 3D imaging of tumor growth
• Echocardiography (including radial and circumferential strain imaging)
• Pulsed-Wave Doppler analysis of blood flow
• Color Doppler analysis of blood flow
• Power Doppler analysis of vascularity

動物生理參數量測 Physiological Measurements in Animals

A full complement of contemporary physiological monitors is available for functional analysis at the systemic level in mice and rats. To ensure compatibility, all physiological signals recorded are acquired, digitized and analyzed by the same software. Radiotelemetry is available to allow physiological measurements from animals in their home cage under conscious condition. Meticulous anesthetic managements based on inhalation of isoflurane or intravenous infusion of propofol are also provided.

• Blood pressure and heart rate measurements
• Baroreflex evaluations based on spectral analysis
• Electrocardiogram (including vector EKG)
• Pressure-volume loop analysis
• Peripheral blood flow measurements (Doppler method)
• Skin blood flow measurements (Doppler method)
• In situ blood flow measurements (e.g. cerebral blood flow)
• Respiratory functions (including rate and volume)
• Blood gas analysis
• Blood cell count
• In situ tissue oxygen and carbon dioxide measurements
• Expiratory oxygen and carbon dioxide measurements
• Metabolic functions (based on metabolic cage)
• Urinary functions
• Drug administration by intravenous, intracerebroventricular, intracisternal, intrathecal route, brain microinjection, and osmotic minipump 
• Small-animal surgery associated with the above physiological measurements or drug administration

動物行為測試 Animal Behavioral Assays

Facilities for measurement and analysis of animal behavior are under development, and a full complement will be available in 2018-2019. A particular feature is that radiotelemetry will be incorporated into some of the behavioral assays to offer information on physiological changes associated with behavior.

• Water maze
• Open field and novel object recognition
• Y-maze
• Six-arm-maze
• Tail suspension
• Passive and active avoidance
• Tail-flick test
• Gait analysis (Catwalk)

疾病動物模式 Animal Models of Diseases

A variety of animal models of diseases has been developed at our Institute, with phenotypes that are compatible with the targeted disease.

Cancer

• Hepatocellular carcinoma
• Oral cancer
• Lung cancer
• Brain glioma
• Brest cancer

Cardiovascular Diseases

• Acute myocardial infarction
• Aortostenosis
• Pressure overload-induced cardiac hypertrophy
• Doxorubicin-induced cardiomyopathy
• Dilated cardiomyopathy
• Arrhythmogenic ventricular cardiomyopathy
• Cardiac hypertrophy
• Chronic myocardial infarction
• Neurogenic hypertension
• DOCA-Salt hypertension
• Ischemic heart disease
• Lower limb ischemia

Lung Diseases

• Ischemia-reperfusion induced acute lung injury
• Hypoxia-induced pulmonary hypertension
• Monocrotaline-induced pulmonary hypertension

Neurological Diseases

• Brain stem death
• Hepatic encephalopathy
• Temporal lobe status epilepticus
• Ischemic stroke
• Aging-associated neurodegenerative diseases

Renal Diseases

• Chronic kidney disease
• Obstructive renal injury
• Ischemia-reperfusion induced kidney injury

Liver Diseases

• Acute liver injury
• Chronic liver injury
• Fatty liver
• Allograft liver transplantation

Others

• Developmental programming
• Sepsis and septic shock
• Organic phosphate poisoning
• Systemic inflammation
• Urinary bladder overactivity
• Tumor metastasis
• Metabolic syndrome

基因轉殖動物 Transgenic Mouse Models

Genetically-manipulated mice acquired or developed by the Institute are available to complement the clinically-relevant animal models.

細胞影像分析 Cell Imaging

Facilities and software for confocal and optical microscopy are available. In particular, our confocal facility has received ISO-17025 accreditation since December, 2014.

• Time-lapse imaging of organelle structures in living cell
• Fluorescence recovery after photobleaching (FRAP)
• Fluorescence loss in photobleaching (FLIP)
• Fluorescence localization after photobleaching (FLAP)
• Fluorescence resonance energy transfer (FREP)
• Fluorescence lifetime imaging microscopy(FLIM)
• Comet assay
• Cell migration/invasion assay
• Wide field high content imaging system (combines automated microscopy with quantitative image analysis to simultaneously quantify multiple functional parameters at single cell level)

組織病理學分析 Histopathological and Histochemical Examinations

Facilities are available for tissue perfusion, frozen and paraffin sectioning, and automatic staining.

• Hematoxylin and eosin (H&E) staining
• Sirius-Red staining
• MTC (Masson’s Trichrome) staining
• TTC (2,3,5-triphenyltetrazolium chloride) staining
• PSA (Periodic acid–Schiff) staining
• Immunohistochemistry
• Single, double or multiple immunofluorescent staining

活細胞與胞器功能量測 Measurements of Cellular and Organelle Functions

We provide advanced technologies to study functions at the cellular and organelle levels.

• Mitochondrial respiratory chain activities (Sea Horse)
• Mitochondrial oxygen consumption
• Glycolysis (Seahorse)
• Mitochondria isolation
• Extracellular vesicle isolation
• Fluorescence imaging of mitochondrial dynamics
• Detection of superoxide by fluorescence probes
• Electron paramagnetic resonance (EPR) detection of superoxide
• Flowcytometry
• Primary cell-culture techniques, including fibroblasts, neurons, astrocytes, hepatocytes, hepatic stellate cells, adipose-derived stem cells, and endothelial cells
• Recombinant protein expression systems in E.Coli and mammalian cells and protein purification
• Monoclonal antibody generation, purification, and functional validation.
• Cell-based luciferase/SEAP reporter assay for screening of cytokine and gene promoter activity.
• ELISA-based assay system for screening of protein-protein interaction.
• Real-time PCR and Western blot analysis
• Immunoprecipitation and immunoblot analysis
• HPLC analysis

基因轉殖與基因分析 Molecular Cloning and Gene analysis

• Mobility shift electrophoresis (EMSA) assay
• Chromatin immunoprecipitation (CHIP) assay
• Vector construction for gene knock-down and over-expression
• Liposome, electroporation, and virus-based gene delivery to cultured cell and animals
• CRISPR/Cas9 technique for genomic editing
• Cancer stem-like cell isolation