聯絡電話
吳芎歷
教授
環境對學習與記憶的影響
飲食對學習與記憶的影響
飲食對腸道菌相及中樞神經功能的影響
- 電子郵件信箱
klhwu@cgmh.org.tw
研究簡介
中樞神經因應外在環境的變動，彙整並調節個體內在的訊息以維持生理功能的恆常運作。飲食是個體獲得能量維持生理功能主要來源，過度飲食損害器官生理功能。我們的研究主題為探討高糖飲食影響學習記憶中樞，海馬廻，導致學習記憶能力缺失的致病機轉。並透過動物模式研究尋求臨床治療與預防最適宜的時機與策略。粒線體為細胞能量代謝的核心，因應細胞外的營養源濃度波動，維持細胞功能。因此粒線體品質控制與恆定維持受到多個機制調控，避免因粒線體失能造成細胞失能，甚至死亡。我們的研究著眼於粒線體功能的恆定與波動因應環境改變所扮演的角色，並將研究理念延伸至臨床應用，與臨床醫師合作探討兒童急性心臟衰竭以及慢性發炎引起腦中風的機轉與治療效益。
研究主題一
探討環境與高果糖飲食影響子代學習與記憶的機轉
經由懷孕及哺乳期餵食高糖飲食的動物模式，我們證實子代學習與記憶能力受損。由環境豐富化刺激，子代學習與記憶能力可回復正常。其中一個重要的因素為星狀膠細胞的能量代謝功能受抑制而限制星狀膠細胞-神經元代謝偶合效應，進而影響神經活性。
研究主題二
探討環境與高果糖飲食影響個體學習與記憶的機轉
我們證實經由餵食高果糖飲食的動物模式，造成個體學習與記憶能力受損。高果糖飲食習慣經由引起神經發炎，降低突觸可塑性以及抑制新生造成學習與記憶能力受損。其中，腸道菌相失衡扮演重要角色。
合作研究主題一
探討急性正腎上腺素上升影響兒童心肺功能的機轉及臨床用藥效益的評估
我們與兒童內科林宜君副部長合作以急性正腎上腺素 (norepinephrine, NE) 上升的動物模式，模擬腸病毒 71 型引起急性心臟衰竭的症狀，研究心肺細胞因應急性正腎上腺素上升，改變細胞分子的生理功能並影響臨床用藥的反應用以提高治療成效。
合作研究主題二
探討發炎影響腦血管自主調控的機轉及對腦中風預後的影響
我們與腦血管科傅睦惠醫師合作以腦中風的動物模式，探索腦中風影響腦血管自主調控，改變神經生理功能的機轉並發展新興療法提高治療效益。
學術著作
Recent publications (Pubmed Search)
1: Lee WC, Lu S, Su CH, Tain YL, Wu KLH, Hsu CN, Tzeng HT. Tadalafil Ameliorates
Chronic Ischemia-Associated Bladder Overactivity in Fructose-Fed Rats by
Exerting Pelvic Angiogenesis and Enhancing p-eNOS Expression. Int J Mol Sci.
2025 Feb 6;26(3):1363. doi: 10.3390/ijms26031363. PMID: 39941129; PMCID:
PMC11818424.
2: Hsu CC, Wu KLH, Peng JM, Wu YN, Chen HT, Lee MS, Cheng JH. Low-energy
extracorporeal shockwave therapy improves locomotor functions, tissue
regeneration, and modulating the inflammation induced FGF1 and FGF2 signaling to
protect damaged tissue in spinal cord injury of rat model: an experimental
animal study. Int J Surg. 2024 Dec 1;110(12):7563-7572. doi:
10.1097/JS9.0000000000002128. PMID: 39453843; PMCID: PMC11634128.
3: Tain YL, Hou CY, Tzeng HT, Lin SF, Chang-Chien GP, Lee WC, Wu KLH, Yu HR,
Chan JYH, Hsu CN. Effect of Purified Resveratrol Butyrate Ester Monomers against
Hypertension after Maternal High-Fructose Intake in Adult Offspring. Nutrients.
2024 Sep 17;16(18):3132. doi: 10.3390/nu16183132. PMID: 39339732; PMCID:
PMC11435219.
4: Huang HM, Chang YC, Lee JJ, Huang YS, Wu KLH. ω-3 PUFAs Ameliorated the
Maternal High-Fructose Diet-Induced Early-Onset Retinopathy in Offspring via
Inhibiting NLRP3-Associated Neuroinflammation. Mol Nutr Food Res. 2024
Oct;68(20):e2400135. doi: 10.1002/mnfr.202400135. Epub 2024 Sep 24. PMID:
39318064.
5: Lin IC, Wu KLH, Cheng HH, Tsai CC, Yu HR, Hsu TY, Tain YL, Huang LT, Lai YJ.
Association of Perinatal Cardiovascular Features with Angiotensin System
Expressions in Maternal Preeclampsia. Int J Mol Sci. 2024 Jul 6;25(13):7426.
doi: 10.3390/ijms25137426. PMID: 39000532; PMCID: PMC11242154.
6: Yu HR, Yeh YT, Tzeng HT, Dai HY, Lee WC, Wu KLH, Chan JYH, Tain YL, Hsu CN.
Carbohydrate-Mediated Pregnancy Gut Microbiota and Neonatal Low Birth Weight.
Nutrients. 2024 Apr 28;16(9):1326. doi: 10.3390/nu16091326. PMID: 38732572;
PMCID: PMC11085476.
7: Wu KLH, Liu WC, Wu CW, Fu MH, Huang HM, Tain YL, Liang CK, Hung CY, Chen IC,
Hung PL, Lin YJ, Hirase H. Butyrate reduction and HDAC4 increase underlie
maternal high fructose-induced metabolic dysfunction in hippocampal astrocytes
in female rats. J Nutr Biochem. 2024 Apr;126:109571. doi:
10.1016/j.jnutbio.2024.109571. Epub 2024 Jan 8. PMID: 38199310.
8: Wu KLH, Wu CW, Chen LW, Chang HH, Cheng CL, Wu CY, Lee YC, Chen IC, Hung CY,
Liu WC. Dysregulation of mitochondrial dynamics mediated aortic perivascular
adipose tissue-associated vascular reactivity impairment under excessive
fructose intake. Nutr Metab (Lond). 2024 Jan 2;21(1):4. doi:
10.1186/s12986-023-00776-7. PMID: 38167066; PMCID: PMC10763079.
9: Yu HR, Tsai CC, Chan JYH, Lee WC, Wu KLH, Tain YL, Hsu TY, Cheng HH, Huang
HC, Huang CH, Pan WH, Yeh YT. A Higher Abundance of Actinomyces spp. in
the Gut Is Associated with Spontaneous Preterm Birth. Microorganisms. 2023 Apr
29;11(5):1171. doi: 10.3390/microorganisms11051171. PMID: 37317145; PMCID:
PMC10222247.
10: Tain YL, Hou CY, Chang-Chien GP, Lin S, Tzeng HT, Lee WC, Wu KLH, Yu HR,
Chan JYH, Hsu CN. Reprogramming Effects of Postbiotic Butyrate and Propionate on
Maternal High-Fructose Diet-Induced Offspring Hypertension. Nutrients. 2023 Mar
30;15(7):1682. doi: 10.3390/nu15071682. PMID: 37049522; PMCID: PMC10096847.
11: Lin IC, Wu CW, Tain YL, Chen IC, Hung CY, Wu KLH. Corrigendum to “High
fructose diet induces early mortality via autophagy factors accumulation in the
rostral ventrolateral medulla as ameliorated by pioglitazone” [J Nutr Biochem
2019;69:87-97]. J Nutr Biochem. 2023 Jun;116:109295. doi:
10.1016/j.jnutbio.2023.109295. Epub 2023 Mar 25. Erratum for: J Nutr Biochem.
2019 Jul;69:87-97. doi: 10.1016/j.jnutbio.2019.03.014. PMID: 36973165.
12: Fu MH, Huang CC, Wu KLH, Chen YF, Kung YC, Lee CC, Liu JS, Lan MY, Chang YY.
Higher prevalence of idiopathic normal pressure hydrocephalus-like MRI features
in progressive supranuclear palsy: An imaging reminder of atypical parkinsonism.
Brain Behav. 2023 Feb;13(2):e2884. doi: 10.1002/brb3.2884. Epub 2023 Jan 12.
PMID: 36635882; PMCID: PMC9927835.
13: Hsu SL, Jhan SW, Hsu CC, Wu YN, Wu KLH, Kuo CA, Chiu HW, Cheng JH. Effect of
three clinical therapies on cytokines modulation in the hip articular cartilage
and bone improvement in rat early osteonecrosis of the femoral head. Biomed J.
2023 Dec;46(6):100571. doi: 10.1016/j.bj.2022.11.004. Epub 2022 Nov 26. PMID:
36442793; PMCID: PMC10749886.
14: Lee WC, Yu HR, Tain YL, Wu KLH, Chuang YC, Chan JYH. Vinpocetine Ameliorates
Metabolic-Syndrome-Associated Bladder Overactivity in Fructose-Fed Rats by
Restoring Succinate-Modulated cAMP Levels and Exerting Anti-Inflammatory Effects
in the Bladder Detrusor Muscle. Biomedicines. 2022 Oct 26;10(11):2716. doi:
10.3390/biomedicines10112716. PMID: 36359236; PMCID: PMC9687486.
15: Liu WC, Wu CW, Fu MH, Tain YL, Liang CK, Chen IC, Hung CY, Lee YC, Wu KLH.
Lipopolysaccharide-induced Autophagy Increases SOX2-positive Astrocytes While
Decreasing Neuronal Differentiation in the Adult Hippocampus. Exp Neurobiol.
2022 Oct 31;31(5):307-323. doi: 10.5607/en22005. PMID: 36351841; PMCID:
PMC9659488.
16: Chao YM, Tain YL, Lee WC, Wu KLH, Yu HR, Chan JYH. Protection by -Biotics
against Hypertension Programmed by Maternal High Fructose Diet: Rectification of
Dysregulated Expression of Short-Chain Fatty Acid Receptors in the Hypothalamic
Paraventricular Nucleus of Adult Offspring. Nutrients. 2022 Oct 14;14(20):4306.
doi: 10.3390/nu14204306. PMID: 36296991; PMCID: PMC9609147.
17: Weng JR, Wu CW, Chen YC, Fu MH, Tain YL, Hung CY, Chen IC, Lee CW, Wu KLH.
Fructose milieu undermines the therapeutic effect of Tribulus terrestris extract
on neuroblastoma cell line via maintaining mitochondrial function. Environ
Toxicol. 2022 Nov;37(11):2728-2742. doi: 10.1002/tox.23632. Epub 2022 Aug 18.
PMID: 36214339.
18: Lee WC, Wu KLH, Tain YL, Leu S, Cheng YT, Chan JYH. Impaired insulin
signaling at the bladder mucosa facilitates metabolic syndrome-associated
bladder overactivity in rats with maternal and post-weaning fructose exposure. J
Formos Med Assoc. 2023 Mar;122(3):258-266. doi: 10.1016/j.jfma.2022.09.013. Epub
2022 Oct 4. PMID: 36207218.
19: Hsu CN, Yu HR, Chan JYH, Lee WC, Wu KLH, Hou CY, Chang-Chien GP, Lin S, Tain
YL. Maternal Acetate Supplementation Reverses Blood Pressure Increase in Male
Offspring Induced by Exposure to Minocycline during Pregnancy and Lactation. Int
J Mol Sci. 2022 Jul 18;23(14):7924. doi: 10.3390/ijms23147924. PMID: 35887270;
PMCID: PMC9319590.
20: Hsu CN, Yu HR, Chan JYH, Wu KLH, Lee WC, Tain YL. The Impact of Gut
Microbiome on Maternal Fructose Intake-Induced Developmental Programming of
Adult Disease. Nutrients. 2022 Feb 28;14(5):1031. doi: 10.3390/nu14051031. PMID:
35268005; PMCID: PMC8912426.
21: Lin IC, Wu CW, Lin YJ, Lo MH, Hsieh KS, Chan JYH, Wu KLH. Milrinone effects
on cardiac mitochondria, hemodynamics, and death in catecholamine-infused rats.
Pediatr Res. 2022 Nov;92(5):1309-1315. doi: 10.1038/s41390-022-01964-6. Epub
2022 Feb 4. PMID: 35121850; PMCID: PMC8814569.
22: Leu S, Wu KLH, Lee WC, Tain YL, Chan JYH. Maternal Fructose Intake
Exacerbates Cardiac Remodeling in Offspring with Ventricular Pressure Overload.
Nutrients. 2021 Sep 18;13(9):3267. doi: 10.3390/nu13093267. PMID: 34579143;
PMCID: PMC8467570.
23: Hung PL, Wu KLH, Chen CJ, Siu KK, Hsin YJ, Wang LJ, Wang FS. Music-Based
Intervention Ameliorates Mecp2-Loss-Mediated Sociability Repression in
Mice through the Prefrontal Cortex FNDC5/BDNF Pathway. Int J Mol Sci.
2021 Jul 2;22(13):7174. doi: 10.3390/ijms22137174. PMID: 34281226; PMCID:
PMC8269182.
24: Chang HH, Lin IC, Wu CW, Hung CY, Liu WC, Wu CY, Cheng CL, Wu KLH. High
fructose induced osteogenic differentiation of human valve interstitial cells
via activating PI3K/AKT/mitochondria signaling. Biomed J. 2022
Jun;45(3):491-503. doi: 10.1016/j.bj.2021.06.008. Epub 2021 Jul 3. PMID:
34229104; PMCID: PMC9421924.
25: Huang HM, Wu CW, Chen IC, Lee YC, Huang YS, Hung CY, Wu KLH. Maternal high-
fructose diet induced early-onset retinopathy via the suppression of synaptic
plasticity mediated by mitochondrial dysfunction. Am J Physiol Endocrinol Metab.
2021 Jun 1;320(6):E1173-E1182. doi: 10.1152/ajpendo.00001.2021. Epub 2021 May
10. PMID: 33969706.
26: Lee WC, Leu S, Wu KLH, Tain YL, Chuang YC, Chan JYH. Tadalafil ameliorates
bladder overactivity by restoring insulin-activated detrusor relaxation via the
bladder mucosal IRS/PI3K/AKT/eNOS pathway in fructose-fed rats. Sci Rep. 2021
Apr 15;11(1):8202. doi: 10.1038/s41598-021-87505-3. PMID: 33859259; PMCID:
PMC8050041.
27: Hsu CN, Chan JYH, Wu KLH, Yu HR, Lee WC, Hou CY, Tain YL. Altered Gut
Microbiota and Its Metabolites in Hypertension of Developmental Origins:
Exploring Differences between Fructose and Antibiotics Exposure. Int J Mol Sci.
2021 Mar 6;22(5):2674. doi: 10.3390/ijms22052674. PMID: 33800916; PMCID:
PMC7961901.
28: Hsu CN, Chan JYH, Yu HR, Lee WC, Wu KLH, Chang-Chien GP, Lin S, Hou CY, Tain
YL. Targeting on Gut Microbiota-Derived Metabolite Trimethylamine to Protect
Adult Male Rat Offspring against Hypertension Programmed by Combined Maternal
High-Fructose Intake and Dioxin Exposure. Int J Mol Sci. 2020 Jul
31;21(15):5488. doi: 10.3390/ijms21155488. PMID: 32752013; PMCID: PMC7432895.
29: Liu WC, Wu CW, Hung PL, Chan JYH, Tain YL, Fu MH, Chen LW, Liang CK, Hung
CY, Yu HR, Chen IC, Wu KLH. Environmental Stimulation Counteracts the
Suppressive Effects of Maternal High-Fructose Diet on Cell Proliferation and
Neuronal Differentiation in the Dentate Gyrus of Adult Female Offspring via
Histone Deacetylase 4. Int J Environ Res Public Health. 2020 Jun 1;17(11):3919.
doi: 10.3390/ijerph17113919. PMID: 32492926; PMCID: PMC7312637.
30: Chao YM, Wu KLH, Tsai PC, Tain YL, Leu S, Lee WC, Chan JYH. Anomalous AMPK-
regulated angiotensin AT1R expression and SIRT1-mediated
mitochondrial biogenesis at RVLM in hypertension programming of offspring to
maternal high fructose exposure. J Biomed Sci. 2020 May 23;27(1):68. doi:
10.1186/s12929-020-00660-z. PMID: 32446297; PMCID: PMC7245869.
31: Fu MH, Chang YY, Lin NH, Yang AW, Chang CC, Liu JS, Peng CH, Wu KLH, Perng
MD, Lan MY. Recessively-Inherited Adult-Onset Alexander Disease Caused by a
Homozygous Mutation in the GFAP Gene. Mov Disord. 2020 Sep;35(9):1662-1667. doi:
10.1002/mds.28099. Epub 2020 May 6. PMID: 32374915.
32: Liu WC, Wu CW, Fu MH, Tain YL, Liang CK, Hung CY, Chen IC, Lee YC, Wu CY, Wu
KLH. Maternal high fructose-induced hippocampal neuroinflammation in the adult
female offspring via PPARγ-NF-κB signaling. J Nutr Biochem. 2020 Jul;81:108378.
doi: 10.1016/j.jnutbio.2020.108378. Epub 2020 Mar 21. PMID: 32330843.
33: Fu MH, Chen IC, Lee CH, Wu CW, Lee YC, Kung YC, Hung CY, Wu KLH. Anti-
neuroinflammation ameliorates systemic inflammation-induced mitochondrial DNA
impairment in the nucleus of the solitary tract and cardiovascular reflex
dysfunction. J Neuroinflammation. 2019 Nov 15;16(1):224. doi:
10.1186/s12974-019-1623-0. PMID: 31729994; PMCID: PMC6858639.
34: Leu S, Wu KLH, Lee WC, Tain YL, Chan JYH. The Impact of Maternal Fructose
Exposure on Angiogenic Activity of Endothelial Progenitor Cells and Blood Flow
Recovery After Critical Limb Ischemia in Rat Offspring. Int J Mol Sci. 2019 May
16;20(10):2429. doi: 10.3390/ijms20102429. PMID: 31100865; PMCID: PMC6566409.
35: Lin IC, Wu CW, Tain YL, Chen IC, Hung CY, Wu KLH. High fructose diet induces
early mortality via autophagy factors accumulation in the rostral ventrolateral
medulla as ameliorated by pioglitazone. J Nutr Biochem. 2019 Jul;69:87-97. doi:
10.1016/j.jnutbio.2019.03.014. Epub 2019 Apr 8. Erratum in: J Nutr Biochem. 2023
Jun;116:109295. doi: 10.1016/j.jnutbio.2023.109295. PMID: 31063919.
36: Suwanjang W, Wu KLH, Prachayasittikul S, Chetsawang B, Charngkaew K.
Mitochondrial Dynamics Impairment in Dexamethasone-Treated Neuronal Cells.
Neurochem Res. 2019 Jul;44(7):1567-1581. doi: 10.1007/s11064-019-02779-4. Epub
2019 Mar 19. PMID: 30888577.
37: Wu CW, Hung CY, Hirase H, Tain YL, Lee WC, Chan JYH, Fu MH, Chen LW, Liu WC,
Liang CK, Ho YH, Kung YC, Leu S, Wu KLH. Pioglitazone reversed the fructose-
programmed astrocytic glycolysis and oxidative phosphorylation of female rat
offspring. Am J Physiol Endocrinol Metab. 2019 Apr 1;316(4):E622-E634. doi:
10.1152/ajpendo.00408.2018. Epub 2019 Jan 22. PMID: 30668149.
38: Hung PL, Hsu MH, Yu HR, Wu KLH, Wang FS. Thyroxin Protects White Matter from
Hypoxic-Ischemic Insult in the Immature Sprague⁻Dawley Rat Brain by Regulating
Periventricular White Matter and Cortex BDNF and CREB Pathways. Int J Mol Sci.
2018 Aug 29;19(9):2573. doi: 10.3390/ijms19092573. PMID: 30158497; PMCID:
PMC6164053.
39: Fu MH, Wu CW, Lee YC, Hung CY, Chen IC, Wu KLH. Nrf2 activation attenuates
the early suppression of mitochondrial respiration due to the α-synuclein
overexpression. Biomed J. 2018 Jun;41(3):169-183. doi: 10.1016/j.bj.2018.02.005.
Epub 2018 Jul 6. PMID: 30080657; PMCID: PMC6138761.
40: Lee WC, Wu KLH, Leu S, Tain YL. Translational insights on developmental
origins of metabolic syndrome: Focus on fructose consumption. Biomed J. 2018
Apr;41(2):96-101. doi: 10.1016/j.bj.2018.02.006. PMID: 29866605; PMCID:
PMC6138777.
41: Tain YL, Lee WC, Wu KLH, Leu S, Chan JYH. Resveratrol Prevents the
Development of Hypertension Programmed by Maternal Plus Post-Weaning High-
Fructose Consumption through Modulation of Oxidative Stress, Nutrient-Sensing
Signals, and Gut Microbiota. Mol Nutr Food Res. 2018 Aug;62(15):e1800066. doi:
10.1002/mnfr.201800066. Epub 2018 Jul 12. PMID: 29710384.
42: Tain YL, Leu S, Lee WC, Wu KLH, Chan JYH. Maternal Melatonin Therapy
Attenuated Maternal High-Fructose Combined with Post-Weaning High-Salt Diets-
Induced Hypertension in Adult Male Rat Offspring. Molecules. 2018 Apr
11;23(4):886. doi: 10.3390/molecules23040886. PMID: 29641494; PMCID: PMC6017187.
43: Tain YL, Wu KLH, Lee WC, Leu S, Chan JYH. Prenatal Metformin Therapy
Attenuates Hypertension of Developmental Origin in Male Adult Offspring Exposed
to Maternal High-Fructose and Post-Weaning High-Fat Diets. Int J Mol Sci. 2018
Apr 3;19(4):1066. doi: 10.3390/ijms19041066. PMID: 29614026; PMCID: PMC5979307.
44: Tain YL, Lee WC, Wu KLH, Leu S, Chan JYH. Maternal High Fructose Intake
Increases the Vulnerability to Post-Weaning High-Fat Diet-Induced Programmed
Hypertension in Male Offspring. Nutrients. 2018 Jan 9;10(1):56. doi:
10.3390/nu10010056. PMID: 29315230; PMCID: PMC5793284.
45: Liu WC, Wu CW, Tain YL, Fu MH, Hung CY, Chen IC, Chen LW, Wu KLH. Oral
pioglitazone ameliorates fructose-induced peripheral insulin resistance and
hippocampal gliosis but not restores inhibited hippocampal adult neurogenesis.
Biochim Biophys Acta Mol Basis Dis. 2018 Jan;1864(1):274-285. doi:
10.1016/j.bbadis.2017.10.017. Epub 2017 Oct 18. PMID: 29054390.
46: Wu KL, Wu CW, Tain YL, Chao YM, Hung CY, Tsai PC, Wang WS, Shih CD. Effects
of high fructose intake on the development of hypertension in the spontaneously
hypertensive rats: the role of AT1R/gp91PHOX signaling in
the rostral ventrolateral medulla. J Nutr Biochem. 2017 Mar;41:73-83. doi:
10.1016/j.jnutbio.2016.11.013. Epub 2016 Dec 5. PMID: 28063367.
47: Chao YM, Tain YL, Leu S, Wu KL, Lee WC, Chan JY. Developmental programming
of the metabolic syndrome: Next-generation sequencing analysis of transcriptome
expression in a rat model of maternal high fructose intake. Sheng Li Xue Bao.
2016 Oct 25;68(5):557-567. PMID: 27778020.
48: Tain YL, Lee WC, Wu KLH, Leu S, Chan JYH. Targeting arachidonic acid pathway
to prevent programmed hypertension in maternal fructose-fed male adult rat
offspring. J Nutr Biochem. 2016 Dec;38:86-92. doi:
10.1016/j.jnutbio.2016.08.006. Epub 2016 Sep 7. PMID: 27732913.
49: Lee WC, Tain YL, Wu KL, Leu S, Chan JY. Maternal Fructose Exposure Programs
Metabolic Syndrome-Associated Bladder Overactivity in Young Adult Offspring. Sci
Rep. 2016 Oct 5;6:34669. doi: 10.1038/srep34669. PMID: 27703194; PMCID:
PMC5050417.
50: Hsu CN, Wu KL, Lee WC, Leu S, Chan JY, Tain YL. Aliskiren Administration
during Early Postnatal Life Sex-Specifically Alleviates Hypertension Programmed
by Maternal High Fructose Consumption. Front Physiol. 2016 Jul 12;7:299. doi:
10.3389/fphys.2016.00299. PMID: 27462279; PMCID: PMC4941125.
51: Wu KLH, Wu CW, Chao YM, Hung CY, Chan JYH. Impaired Nrf2 regulation of
mitochondrial biogenesis in rostral ventrolateral medulla on hypertension
induced by systemic inflammation. Free Radic Biol Med. 2016 Aug;97:58-74. doi:
10.1016/j.freeradbiomed.2016.05.012. Epub 2016 May 17. PMID: 27223823.
52: Wu KL, Wu CW, Tain YL, Huang LT, Chao YM, Hung CY, Wu JC, Chen SR, Tsai PC,
Chan JY. Environmental stimulation rescues maternal high fructose intake-
impaired learning and memory in female offspring: Its correlation with
redistribution of histone deacetylase 4. Neurobiol Learn Mem. 2016
Apr;130:105-17. doi: 10.1016/j.nlm.2016.02.004. Epub 2016 Feb 9. PMID: 26872592.
53: Tain YL, Wu KL, Lee WC, Leu S, Chan JY. Maternal fructose-intake-induced
renal programming in adult male offspring. J Nutr Biochem. 2015
Jun;26(6):642-50. doi: 10.1016/j.jnutbio.2014.12.017. Epub 2015 Feb 26. PMID:
25765514.
54: Wu KL, Chao YM, Tsay SJ, Chen CH, Chan SH, Dovinova I, Chan JY. Role of
nitric oxide synthase uncoupling at rostral ventrolateral medulla in redox-
sensitive hypertension associated with metabolic syndrome. Hypertension. 2014
Oct;64(4):815-24. doi: 10.1161/HYPERTENSIONAHA.114.03777. Epub 2014 Jun 23.
PMID: 24958506.
55: Tain YL, Leu S, Wu KL, Lee WC, Chan JY. Melatonin prevents maternal fructose
intake-induced programmed hypertension in the offspring: roles of nitric oxide
and arachidonic acid metabolites. J Pineal Res. 2014 Aug;57(1):80-9. doi:
10.1111/jpi.12145. Epub 2014 Jun 16. PMID: 24867192.
56: Wu KL, Hung CY, Chan JY, Wu CW. An increase in adenosine-5′-triphosphate
(ATP) content in rostral ventrolateral medulla is engaged in the high fructose
diet-induced hypertension. J Biomed Sci. 2014 Jan 27;21(1):8. doi:
10.1186/1423-0127-21-8. PMID: 24467657; PMCID: PMC3913325.
57: Wu KL, Chan SH, Chan JY. Neuroinflammation and oxidative stress in rostral
ventrolateral medulla contribute to neurogenic hypertension induced by systemic
inflammation. J Neuroinflammation. 2012 Sep 7;9:212. doi:
10.1186/1742-2094-9-212. PMID: 22958438; PMCID: PMC3462714.
58: Wu KL, Chen CH, Shih CD. Nontranscriptional activation of PI3K/Akt signaling
mediates hypotensive effect following activation of estrogen receptor β in the
rostral ventrolateral medulla of rats. J Biomed Sci. 2012 Aug 16;19(1):76. doi:
10.1186/1423-0127-19-76. PMID: 22897791; PMCID: PMC3438069.
59: Wu KL, Wu CA, Wu CW, Chan SH, Chang AY, Chan JY. Redox-sensitive oxidation
and phosphorylation of PTEN contribute to enhanced activation of PI3K/Akt
signaling in rostral ventrolateral medulla and neurogenic hypertension in
spontaneously hypertensive rats. Antioxid Redox Signal. 2013 Jan 1;18(1):36-50.
doi: 10.1089/ars.2011.4457. Epub 2012 Aug 16. PMID: 22746319; PMCID: PMC3503464.
60: Chan SH, Wu KL, Kung PS, Chan JY. Oral intake of rosiglitazone promotes a
central antihypertensive effect via upregulation of peroxisome proliferator-
activated receptor-gamma and alleviation of oxidative stress in rostral
ventrolateral medulla of spontaneously hypertensive rats. Hypertension. 2010
Jun;55(6):1444-53. doi: 10.1161/HYPERTENSIONAHA.109.149146. Epub 2010 Apr 19.
PMID: 20404217.
61: Chan SH, Wu CA, Wu KL, Ho YH, Chang AY, Chan JY. Transcriptional
upregulation of mitochondrial uncoupling protein 2 protects against oxidative
stress-associated neurogenic hypertension. Circ Res. 2009 Oct 23;105(9):886-96.
doi: 10.1161/CIRCRESAHA.109.199018. Epub 2009 Sep 17. PMID: 19762685.
62: Wu KL, Hsu C, Chan JY. Nitric oxide and superoxide anion differentially
activate poly(ADP-ribose) polymerase-1 and Bax to induce nuclear translocation
of apoptosis-inducing factor and mitochondrial release of cytochrome c after
spinal cord injury. J Neurotrauma. 2009 Jul;26(7):965-77. doi:
10.1089/neu.2008.0692. PMID: 19473058.
63: Chan SH, Wu KL, Chang AY, Tai MH, Chan JY. Oxidative impairment of
mitochondrial electron transport chain complexes in rostral ventrolateral
medulla contributes to neurogenic hypertension. Hypertension. 2009
Feb;53(2):217-27. doi: 10.1161/HYPERTENSIONAHA.108.116905. Epub 2008 Dec 29.
PMID: 19114648.
64: Kung LC, Chan SH, Wu KL, Ou CC, Tai MH, Chan JY. Mitochondrial respiratory
enzyme complexes in rostral ventrolateral medulla as cellular targets of nitric
oxide and superoxide interaction in the antagonism of antihypertensive action of
eNOS transgene. Mol Pharmacol. 2008 Nov;74(5):1319-32. doi:
10.1124/mol.108.048793. Epub 2008 Aug 20. PMID: 18715945.
65: Wu KL, Hsu C, Chan JY. Impairment of the mitochondrial respiratory enzyme
activity triggers sequential activation of apoptosis-inducing factor-dependent
and caspase-dependent signaling pathways to induce apoptosis after spinal cord
injury. J Neurochem. 2007 Jun;101(6):1552-66. doi:
10.1111/j.1471-4159.2006.04445.x. Epub 2007 Feb 9. PMID: 17298387.
66: Chan SH, Wu KL, Wang LL, Chan JY. Nitric oxide- and superoxide-dependent
mitochondrial signaling in endotoxin-induced apoptosis in the rostral
ventrolateral medulla of rats. Free Radic Biol Med. 2005 Sep 1;39(5):603-18.
doi: 10.1016/j.freeradbiomed.2005.04.012. PMID: 16085179.
67: Tai MH, Wang LL, Wu KL, Chan JY. Increased superoxide anion in rostral
ventrolateral medulla contributes to hypertension in spontaneously hypertensive
rats via interactions with nitric oxide. Free Radic Biol Med. 2005 Feb
15;38(4):450-62. doi: 10.1016/j.freeradbiomed.2004.11.015. PMID: 15649647.
68: Wu KL, Chan SH, Chao YM, Chan JY. Expression of pro-inflammatory cytokine
and caspase genes promotes neuronal apoptosis in pontine reticular formation
after spinal cord transection. Neurobiol Dis. 2003 Oct;14(1):19-31. doi:
10.1016/s0969-9961(03)00078-0. PMID: 13678663.
研究團隊
|
洪純瑛研究助理
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陳怡君研究助理
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蔡裴家研究助理
|
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楊家齊研究助理
|
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林宜君副部長研究團隊
傅睦惠醫師研究團隊
學術履歷
Education
| 2002-2007 | Ph D
The Graduate Institute of Medicine, Kaohsiung Medical University, Taiwan, R.O.C. |
| 1997-1999 | M. S. (June, 1999)
Institute of Physiology in National Yang-Ming University, Taiwan, R.O.C. |
| 1992-1997 | D.V.M. (June, 1997)
College of Veterinary Medicine in National Chung-Hsing University, Taiwan, R.O.C. |
Honors
| 2019 | Invited speaker of Physiology Speaker Travel Agenda of LSUHSC |
| 2018 | 2018 Frontiers in Translational Medicine invited speaker |
| 2017 | BIT’s 9th Annual International Congress of Cardiology-2017 (ICC-2017) invited speaker and Co-chair |
| 2017 | International Conference on Diabetes and its Complications 2017 (ICDC 2017) invited speaker (Poster Section) |
| 2015 | 30th Annual Conference of Biomedical Sciences-Physiology invited speaker |
| 2013 | IUPS 2013 Travel Awards. The title of my presentation was: Fructose increases ATP-and ROS-dependent neurotransmitter release |
| 2011 | Young Investigator Award of the 7th FAOPS Congress.
The title of my presentation was: cyclooxygenase-2-dependent neuroinflammation and oxidative stress in rostral ventrolateral medulla contribute to neurogenic hypertension following peripheral inflammation |
| 2006 | Distinguished Neuroscience Award of Taiwan Neuroscience Society Annual Meeting International Stroke Symposium, Taiwan.
The title of my presentation was: Superoxide anion- and nitric oxide-dependent mitochondrial dysfunction in neuronal apoptosis after spinal cord injury The second runner up poster presentation of the 21th annual Meeting of Biological sciences, Taiwan. The title of my presentation was: Superoxide anion- and nitric oxide-associated mitochondrial dysfunction in neuronal apoptosis after spinal cord injury |
| 2005- | The member of International Brain Research Organization (IBRO) |
| 2003-2006 | Outstanding Graduated Students in the Graduate program of Medicine, Kaohsiung Medicine University. |
| 2000 | The best poster presentation of the Neuroscience Meeting 2000, Taiwan. The title of my presentation was: Neuronal apoptosis in pontine reticular formation after spinal cord transection and the protection by brain-derived neuro-trophic factor. |
| 1997-1999 | Outstanding Graduated Students Scholarship Supported by Ministry of Education |
| 1994 | The best poster presentation of the Fourth Symposium on Recent Advances in Cellular and Molecular Biology, Taiwan. The title of my presentation was: Analysis of unregulated cellular genes in pseudorabies virus infection: use of mRNA differential display |
Research Experiences
- Aug, 2021-
Name of Institute:
Institute for Translational Research in Biomedine, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung, Taiwan, Republic of China.
Job Title: Professor (Hospital)/ Associate Professor (College)
- Aug, 2015-July, 2021
Name of Institute:
Institute for Translational Research in Biomedine, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung, Taiwan, Republic of China.
Job Title: Associate Professor (Hospital)/ Assistant Professor (College)
- June, 2011-July, 2015
Name of Institute:
Center for Translational Research in Biomedical Sciences, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung, Taiwan, Republic of China.
Job Title: Assistant Professor
- September, 2008- May, 2011
Name of Institute:
Neural Structure & Function Research Laboratory, Department of Medical Education and Research,
Veterans General Hospital-Kaohsiung.
Job Title: postdoctoral fellow
Supervisor: Prof. Julie Y. H. Chan
- September, 2007-2008
Name of Institute:
Center for Neuroscience, National Sun Yat-sen University.
Job Title: postdoctoral fellow
Supervisor: Prof. Samuel H. H. Chan
- September, 2002-2007
Name of Institute:
- The Graduate Institute of Medicine, Kaohsiung Medical University.
- Neural Structure & Function Research Laboratory, Department of Medical Education and Research, Veterans General Hospital-Kaohsiung.
Job Title: Ph D student
Supervisor: Prof. Julie Y. H. Chan and Prof. Chin Hsu
- September, 1999 –2002
Name of Institute:
Neural Structure & Function Research Laboratory, Department of Medical Education and Research,
Veterans General Hospital-Kaohsiung.
Job Title: Research technologist
Supervisor: Prof. Julie Y. H. Chan
Project
Protective role of brain-derived neurotrophic factor in neuronal cell death of pontine reticular formation after spinal cord injury.
- March, 1993 –1997
Name of Institute:
Molecular Biology & Veterinary Virology Laboratory,
College of Veterinary Medicine,
National Chung-Hsing University.
Job Title: Research technologist
Supervisor: Dr. Tien-Jye Chang
Project
- Study of early genes on pseudorabies virus expression, gene regulation, and biological functions.
- Construction of a gene-deleted mutant or a replication-deficient virus as an expression vector for foreign genes or a live virus vaccine.