{"id":2149,"date":"2020-02-06T15:49:09","date_gmt":"2020-02-06T07:49:09","guid":{"rendered":"https:\/\/itrbm.org\/en\/?page_id=2149"},"modified":"2020-03-02T16:04:28","modified_gmt":"2020-03-02T08:04:28","slug":"other-research-resources","status":"publish","type":"page","link":"https:\/\/itrbm.org\/en\/imaging-facility\/other-research-resources\/","title":{"rendered":"Other Research Resources"},"content":{"rendered":"<div id='av_section_1' class='avia-section main_color avia-section-large avia-no-border-styling avia-full-stretch av-section-color-overlay-active avia-bg-style-scroll  avia-builder-el-0  el_before_av_one_full  avia-builder-el-first   container_wrap sidebar_left' style='background-repeat: no-repeat; background-image: url(https:\/\/itrbm.org\/en\/wp-content\/uploads\/2018\/01\/dna-2.jpg);background-attachment: scroll; background-position: center center;  '  data-section-bg-repeat='stretch'><div class='av-section-color-overlay-wrap'><div class='av-section-color-overlay' style='opacity: 0.2; background-color: #000000; '><\/div><div class='container' ><main  role=\"main\" itemprop=\"mainContentOfPage\"  class='template-page content  av-content-small units'><div class='post-entry post-entry-type-page post-entry-2149'><div class='entry-content-wrapper clearfix'>\n<div style='padding-bottom:10px; color:#ffffff;font-size:44px;' class='av-special-heading av-special-heading-h1 custom-color-heading blockquote modern-quote modern-centered  avia-builder-el-1  avia-builder-el-no-sibling  av-inherit-size '><h1 class='av-special-heading-tag '  itemprop=\"headline\"  >Other Research Resources<\/h1><div class='special-heading-border'><div class='special-heading-inner-border' style='border-color:#ffffff'><\/div><\/div><\/div>\n<\/div><\/div><\/main><!-- close content main element --><\/div><\/div><\/div><div id='after_section_1' class='main_color av_default_container_wrap container_wrap sidebar_left' style=' '  ><div class='container' ><div class='template-page content  av-content-small units'><div class='post-entry post-entry-type-page post-entry-2149'><div class='entry-content-wrapper clearfix'><div class=\"flex_column av_one_full  flex_column_div av-zero-column-padding first  avia-builder-el-2  el_after_av_section  avia-builder-el-no-sibling  \" style='border-radius:0px; '><section class=\"av_textblock_section \"  itemscope=\"itemscope\" itemtype=\"https:\/\/schema.org\/CreativeWork\" ><div class='avia_textblock  '   itemprop=\"text\" ><blockquote>\n<h3><strong>\u52d5\u7269\u5f71\u50cf\u7cfb\u7d71 Animal Imaging<br \/>\n<\/strong><\/h3>\n<\/blockquote>\n<div>\n<p><span style=\"font-size: medium;\">We offer a variety of preclinical imaging facilities, including 9.4T animal MRI, bioluminescence and fluorescence imaging system (IVIS), and animal ultrasound imaging system.<\/span><\/p>\n<ul>\n<li>Diffusion MRI (including diffusion tensor imaging and diffusion weighted imaging)<\/li>\n<li>Functional MRI and BOLD MR imaging<\/li>\n<li>Chemical exchange saturation transfer (CEST) MRI<\/li>\n<li>MR spectrum for metabolites (including  1 H and X nuclear)<\/li>\n<li>Wideband magnetic resonance imaging (WB-MRI) for high spatio-temporal MR imaging<\/li>\n<li>Quantitative susceptibility mapping (QSM) algorithm for quantifying magnetic properties and<br \/>\novercome non-locality of the magnetic field distribution from phase data<\/li>\n<li>In vivo optical imaging, including bioluminescence and fluorescence imaging<\/li>\n<li>2D or 3D imaging of tumor growth<\/li>\n<li>Echocardiography (including radial and circumferential strain imaging)<\/li>\n<li>Pulsed-Wave Doppler analysis of blood flow<\/li>\n<li>Color Doppler analysis of blood flow<\/li>\n<li>Power Doppler analysis of vascularity<\/li>\n<\/ul>\n<blockquote>\n<h3><strong>\u52d5\u7269\u751f\u7406\u53c3\u6578\u91cf\u6e2c Physiological Measurements in Animals<\/strong><\/h3>\n<\/blockquote>\n<p><span style=\"font-size: medium;\">A full complement of contemporary physiological monitors is available for functional analysis at the systemic level in mice and rats. To ensure compatibility, all physiological signals recorded are acquired, digitized and analyzed by the same software. Radiotelemetry is available to allow physiological measurements from animals in their home cage under conscious condition. Meticulous anesthetic managements based on inhalation of isoflurane or intravenous infusion of propofol are also provided.<\/span><\/p>\n<ul>\n<li>Blood pressure and heart rate measurements<\/li>\n<li>Baroreflex evaluations based on spectral analysis<\/li>\n<li>Electrocardiogram (including vector EKG)<\/li>\n<li>Pressure-volume loop analysis<\/li>\n<li>Peripheral blood flow measurements (Doppler method)<\/li>\n<li>Skin blood flow measurements (Doppler method)<\/li>\n<li>In situ blood flow measurements (e.g. cerebral blood flow)<\/li>\n<li>Respiratory functions (including rate and volume)<\/li>\n<li>Blood gas analysis<\/li>\n<li>Blood cell count<\/li>\n<li>In situ tissue oxygen and carbon dioxide measurements<\/li>\n<li>Expiratory oxygen and carbon dioxide measurements<\/li>\n<li>Metabolic functions (based on metabolic cage)<\/li>\n<li>Urinary functions<\/li>\n<li>Drug administration by intravenous, intracerebroventricular, intracisternal, intrathecal route, brain microinjection, and osmotic minipump \uf0b7<\/li>\n<li>Small-animal surgery associated with the above physiological measurements or drug administration<\/li>\n<\/ul>\n<blockquote>\n<h3><strong>\u52d5\u7269\u884c\u70ba\u6e2c\u8a66 Animal Behavioral Assays<\/strong><\/h3>\n<\/blockquote>\n<p><span style=\"font-size: medium;\">Facilities for measurement and analysis of animal behavior are under development, and a full complement will be available in 2018-2019. A particular feature is that radiotelemetry will be incorporated into some of the behavioral assays to offer information on physiological changes associated with behavior.<\/span><\/p>\n<ul>\n<li>Water maze<\/li>\n<li>Open field and novel object recognition<\/li>\n<li>Y-maze<\/li>\n<li>Six-arm-maze<\/li>\n<li>Tail suspension<\/li>\n<li>Passive and active avoidance<\/li>\n<li>Tail-flick test<\/li>\n<li>Gait analysis (Catwalk)<\/li>\n<\/ul>\n<blockquote>\n<h3><strong>\u75be\u75c5\u52d5\u7269\u6a21\u5f0f Animal Models of Diseases<\/strong><\/h3>\n<\/blockquote>\n<p><span style=\"font-size: medium;\">A variety of animal models of diseases has been developed at our Institute, with phenotypes that are compatible with the targeted disease.<\/span><\/p>\n<p><strong>Cancer <\/strong><\/p>\n<ul>\n<li>Hepatocellular carcinoma<\/li>\n<li>Oral cancer<\/li>\n<li>Lung cancer<\/li>\n<li>Brain glioma\uf0b7<\/li>\n<li>Brest cancer<\/li>\n<\/ul>\n<p><b>Cardiovascular Diseases<\/b><\/p>\n<ul>\n<li>Acute myocardial infarction<\/li>\n<li>Aortostenosis<\/li>\n<li>Pressure overload-induced cardiac hypertrophy<\/li>\n<li>Doxorubicin-induced cardiomyopathy<\/li>\n<li>Dilated cardiomyopathy<\/li>\n<li>Arrhythmogenic ventricular cardiomyopathy<\/li>\n<li>Cardiac hypertrophy<\/li>\n<li>Chronic myocardial infarction<\/li>\n<li>Neurogenic hypertension<\/li>\n<li>DOCA-Salt hypertension<\/li>\n<li>Ischemic heart disease<\/li>\n<li>Lower limb ischemia<\/li>\n<\/ul>\n<p><b>Lung Diseases<\/b><\/p>\n<ul>\n<li>Ischemia-reperfusion induced acute lung injury<\/li>\n<li>Hypoxia-induced pulmonary hypertension<\/li>\n<li>Monocrotaline-induced pulmonary hypertension<\/li>\n<\/ul>\n<p><b>Neurological Diseases<\/b><\/p>\n<ul>\n<li>Brain stem death<\/li>\n<li>Hepatic encephalopathy<\/li>\n<li>Temporal lobe status epilepticus<\/li>\n<li>Ischemic stroke<\/li>\n<li>Aging-associated neurodegenerative diseases<\/li>\n<\/ul>\n<p><b>Renal Diseases<\/b><\/p>\n<ul>\n<li>Chronic kidney disease<\/li>\n<li>Obstructive renal injury<\/li>\n<li>Ischemia-reperfusion induced kidney injury<\/li>\n<\/ul>\n<p><b>Liver Diseases<\/b><\/p>\n<ul>\n<li>Acute liver injury<\/li>\n<li>Chronic liver injury<\/li>\n<li>Fatty liver<\/li>\n<li>Allograft liver transplantation<\/li>\n<\/ul>\n<p><b>Others <\/b><\/p>\n<ul>\n<li>Developmental programming<\/li>\n<li>Sepsis and septic shock<\/li>\n<li>Organic phosphate poisoning<\/li>\n<li>Systemic inflammation<\/li>\n<li>Urinary bladder overactivity<\/li>\n<li>Tumor metastasis<\/li>\n<li>Metabolic syndrome<\/li>\n<\/ul>\n<blockquote>\n<h3><strong>\u57fa\u56e0\u8f49\u6b96\u52d5\u7269 Transgenic Mouse Models<\/strong><\/h3>\n<\/blockquote>\n<p><span style=\"font-size: medium;\">Genetically-manipulated mice acquired or developed by the Institute are available to complement the clinically-relevant animal models.<\/span><\/p>\n<blockquote>\n<h3><strong>\u7d30\u80de\u5f71\u50cf\u5206\u6790 Cell Imaging<\/strong><\/h3>\n<\/blockquote>\n<p><span style=\"font-size: medium;\">Facilities and software for confocal and optical microscopy are available. In particular, our confocal facility has received ISO-17025 accreditation since December, 2014.<\/span><\/p>\n<ul>\n<li>Time-lapse imaging of organelle structures in living cell<\/li>\n<li>Fluorescence recovery after photobleaching (FRAP)<\/li>\n<li>Fluorescence loss in photobleaching (FLIP)<\/li>\n<li>Fluorescence localization after photobleaching (FLAP)<\/li>\n<li>Fluorescence resonance energy transfer (FREP)<\/li>\n<li>Fluorescence lifetime imaging microscopy(FLIM)<\/li>\n<li>Comet assay<\/li>\n<li>Cell migration\/invasion assay<\/li>\n<li>Wide field high content imaging system (combines automated microscopy with quantitative image analysis to simultaneously quantify multiple functional parameters at single cell level)<\/li>\n<\/ul>\n<blockquote>\n<h3><strong>\u7d44\u7e54\u75c5\u7406\u5b78\u5206\u6790 Histopathological and Histochemical Examinations<\/strong><\/h3>\n<\/blockquote>\n<p><span style=\"font-size: medium;\">Facilities are available for tissue perfusion, frozen and paraffin sectioning, and automatic staining.<\/span><\/p>\n<ul>\n<li>Hematoxylin and eosin (H&#038;E) staining<\/li>\n<li>Sirius-Red staining<\/li>\n<li>MTC (Masson\u2019s Trichrome) staining<\/li>\n<li>TTC (2,3,5-triphenyltetrazolium chloride) staining<\/li>\n<li>PSA (Periodic acid\u2013Schiff) staining<\/li>\n<li>Immunohistochemistry<\/li>\n<li>Single, double or multiple immunofluorescent staining<\/li>\n<\/ul>\n<blockquote>\n<h3><strong>\u6d3b\u7d30\u80de\u8207\u80de\u5668\u529f\u80fd\u91cf\u6e2c Measurements of Cellular and Organelle Functions<\/strong><\/h3>\n<\/blockquote>\n<p><span style=\"font-size: medium;\">We provide advanced technologies to study functions at the cellular and organelle levels.<\/span><\/p>\n<ul>\n<li>Mitochondrial respiratory chain activities (Sea Horse)<\/li>\n<li>Mitochondrial oxygen consumption<\/li>\n<li>Glycolysis (Seahorse)<\/li>\n<li>Mitochondria isolation<\/li>\n<li>Extracellular vesicle isolation<\/li>\n<li>Fluorescence imaging of mitochondrial dynamics<\/li>\n<li>Detection of superoxide by fluorescence probes<\/li>\n<li>Electron paramagnetic resonance (EPR) detection of superoxide<\/li>\n<li>Flowcytometry<\/li>\n<li>Primary cell-culture techniques, including fibroblasts, neurons, astrocytes, hepatocytes, hepatic stellate cells, adipose-derived stem cells, and endothelial cells<\/li>\n<li>Recombinant protein expression systems in E.Coli and mammalian cells and protein purification<\/li>\n<li>Monoclonal antibody generation, purification, and functional validation.<\/li>\n<li>Cell-based luciferase\/SEAP reporter assay for screening of cytokine and gene promoter activity.<\/li>\n<li>ELISA-based assay system for screening of protein-protein interaction.<\/li>\n<li>Real-time PCR and Western blot analysis<\/li>\n<li>Immunoprecipitation and immunoblot analysis<\/li>\n<li>HPLC analysis<\/li>\n<\/ul>\n<blockquote>\n<h3><strong>\u57fa\u56e0\u8f49\u6b96\u8207\u57fa\u56e0\u5206\u6790 Molecular Cloning and Gene analysis<\/strong><\/h3>\n<\/blockquote>\n<ul>\n<li>Mobility shift electrophoresis (EMSA) assay<\/li>\n<li>Chromatin immunoprecipitation (CHIP) assay<\/li>\n<li>Vector construction for gene knock-down and over-expression<\/li>\n<li>Liposome, electroporation, and virus-based gene delivery to cultured cell and animals<\/li>\n<li>CRISPR\/Cas9 technique for genomic editing<\/li>\n<li>Cancer stem-like cell isolation<\/li>\n<\/ul>\n<\/div>\n<\/div><\/section><\/div><\/p>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":2,"featured_media":0,"parent":462,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"_links":{"self":[{"href":"https:\/\/itrbm.org\/en\/wp-json\/wp\/v2\/pages\/2149"}],"collection":[{"href":"https:\/\/itrbm.org\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/itrbm.org\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/itrbm.org\/en\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/itrbm.org\/en\/wp-json\/wp\/v2\/comments?post=2149"}],"version-history":[{"count":4,"href":"https:\/\/itrbm.org\/en\/wp-json\/wp\/v2\/pages\/2149\/revisions"}],"predecessor-version":[{"id":2325,"href":"https:\/\/itrbm.org\/en\/wp-json\/wp\/v2\/pages\/2149\/revisions\/2325"}],"up":[{"embeddable":true,"href":"https:\/\/itrbm.org\/en\/wp-json\/wp\/v2\/pages\/462"}],"wp:attachment":[{"href":"https:\/\/itrbm.org\/en\/wp-json\/wp\/v2\/media?parent=2149"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}